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While it is well established that metals are highly toxic for multiple organs and systems through inhalation, no studies have evaluated the specific health effects of metals in e-cigarettes, except in the study of copper nanoparticles from e-cigarettes and mitochondrial oxidative stress and DNA fragmentation

The conversion rate of nicotine to nornicotine is controlled by particular genes in the plant but is not consistent from plant to plant, and declines in successive generations

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