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J Endotoxin Res 8:319327, doi:10.1177/09680519020080050501 Lu SC, Ge JL (1992) Loss of suppression of GSH synthesis at low cell density in primary cultures of rat hepatocytes

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Tumours were cut into small pieces (15 mm), put into the enzyme mix in RPMI and dissociated using gentleMACS Octo Dissociator with Heaters (Miltenyi) with the appropriate gentleMACS program (37C_m_TDK)

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Individuals with severe MTHFR deficiency are characterised by highly impaired production of 5-MTHF

RT-PCR, real-time reverse-transcriptase-polymerase chain reaction